Vol. 1, Issue 6 (2013)
Antioxidant Activity of Centella asiatica (Linn.) Urban: Impact of Extraction Solvent Polarity
Author(s): Mijanur Rahman , Shahdat Hossain *, Asiqur Rahaman , Nusrat Fatima , Taslima Nahar, Borhan Uddin and Mafroz Ahmed Basunia
Abstract: In vitro antioxidant activity of Centella asiatica (Linn.) and the impact of extraction solvent polarity on the antioxidant potential were investigated in the present study. 100% ethanol, 50% ethanol and water were chosen as extraction solvent due to arithmetic progression of their polarity. Total polyphenol, flavonoid, β-carotene, tannin and vitamin C content of three extracts were estimated while antioxidant potential was assayed by total reducing power assay and 2, 2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging activity. 50% ethanol extract of C. asiatica contain significantly higher amount of polyphenol, flavonoid while moderate amount of carotene and tannin but lowest vitamin C compared to 100% ethanol and water extract. All the phytochemicals showed solvent polarity specific extraction pattern. Total reducing power and DPPH-radical scavenging activity of 50% ethanol extract were significantly higher compared to 100% ethanol and water extract. Significant variation of antioxidant potential of C. asiatica due to extraction solvent polarity difference was demonstrated from this study.
How to cite this article:
Mijanur Rahman , Shahdat Hossain *, Asiqur Rahaman , Nusrat Fatima , Taslima Nahar, Borhan Uddin and Mafroz Ahmed Basunia. Antioxidant Activity of Centella asiatica (Linn.) Urban: Impact of Extraction Solvent Polarity. Journal of Pharmacognosy and Phytochemistry. 2013; 1(6): 27-32.