Author(s):

Sami G. Alsabri, Nouri B. Rmeli, Abdulmottaleb A. Zetrini, Salah B. Mohamed, Marwa I. Meshri, Khaled M. Aburas, Salah M. Bensaber, Ibrahim A. Mrema, Akram A. Mosbah, Khowla A. Allahresh , Anton Hermann4 and Abdul Gbaj

Abstract: The medicinal plants have been considered a healthy source of life for all people and the therapeutical properties of the medical plants are very useful in curing range of diseases. The aims of the study are to evaluate the anti-oxidant, anti-microbial, anti-inflammatory and anti-ulcer properties of Helianthemum lippii (MHL) methanolic extracts. MHL samples were collected in the mountain region of Libya. The antioxidant properties of MHL were evaluated using free radical scavenging assay. Stomach ulcers were induced in rats by ethanol. Pretreatment with ranitidine and MHL samples were performed before the ulcer induction. Gastric mucosal histological changes in rat stomach tissue were evaluated. Antimicrobial efficacy of MHL was also studied against gram-positive bacteria S. aureus, gram-negative bacteria E. coli , and fungal strain C. albicans. The phytochemical screening of MHL showed presence of flavonoids, tannins, saponine and simple phenolic compounds. MHL exhibited a powerful anti-oxidant activity, where 31.17 ± 1.40 µg/ml of extract caused 50% inhibition on 2,2, Diphenyl-1-picryhydrazyl (DPPH) scavenging activity in comparison to standard ascorbic acid (15.35 ± 3.2 µg/ml). MHL also showed substantial anti-microbial activity against a strain of gram-positive bacteria, S. aureu, with zone of inhibition (21 mm) and MIC (12.25 mg/ml) and a fungal strain, C. albicans, with zone of inhibition (20 mm) and MIC (6.25 mg/ml) compared to ciprofloxacin or amphotericin B. MHL at doses of 250 and 500 mg/kg produced statistical significant anti-inflammatory activity (23.6%, 50%) in comparison to aspirin (60%). It further showed significant anti-ulcer activity in doses of 250mg/kg and 500mg/kg with percentage of gastric lesions inhibitions of 48.78% and 76.82%, respectively, in comparison to standard anti-ulcer ranitidine (50 mg/kg), which showed 69.78%. MHL has clearly a protective effect against ethanol-induced gastric mucosal lesion, and this effect, at least in part, depends on the increase of anti-oxidant activity. In conclusion, the MHL extract showed anti-oxidant, anti-ulcer, anti-microbial activities against tested microbes, antioxidant and anti-inflammatory; mightily due to combined mechanisms of MHL’s constituents.rn

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