Vol. 2, Issue 2, Part B (2013)
Comparative Phytochemical and Pharmacological Evaluations of Two Varieties of Ocimum basilicum for Antiarthritic Activity
Author(s): Sapna Phadtare, Rahul Pandit, Vaibhav Shinde, Kakasaheb Mahadik
Abstract: Tulsi plants (family Lamiaceae) are widely used in the Ayurvedic system of medicine for bronchitis, bronchial asthma, skin diseases, arthritis, inflammation, fever etc. The study was carried out to corroborate the traditional claims for anti-arthritic activity of tulsi. A comparative study of two varieties of O.basilicum, basilicum and thyrsiflora was done for evaluation of anti-inflammatory and anti-arthritic activity. Carrageenan induced paw oedema for anti-inflammatory activity and Complete Freund’s Adjuvent induced arthritis for anti-arthritic activity were the two animal models used for the investigation. The present investigation suggests that O.basilicum var.basilicum (OSBB) at a dose of 50,100 & 200mg/kg p.o. exhibits decrease in carrageenan induced rat paw oedema. OSBB possess potent anti-arthritic and anti-inflammatory activity in a dose dependent manner. In case of arthritis there was significant inhibition at the dose of 100 & 200 mg/kg (p less than 0.01 and 0.001 respectively). O.basilicum var.thyrsiflora (OSBT: p less than 0.05) was unable to show activity at lower dose. Thus higher dose of OSBT may be required to exhibit the same pattern of anti-arthritic and anti-inflammatory activity in lab animals. The study was carried out to corroborate the traditional claims for anti-arthritic activity as well as quantification of three marker compounds ursolic acid, oleanolic acid and eugenol by HPTLC and HPLC and to check interspecies variation.
Graph of effect of Ocimum basilicum var.thyrsiflorum on joint diameter