Pharmacognosy

Journal of Pharmacognosy and Phytochemistry

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Journal of Pharmacognosy and Phytochemistry

Vol. 2, Issue 6 (2014)

High frequency plant regeneration from shoot tip explants of Citrullus colocynthis (Linn.) Schrad. –An important medicinal herb

Author(s): Mahesh Chand Meena, Rishi Kesh Meena and Vidya Patni
Abstract: A high frequency and rapid regeneration protocol was developed from shoot tip explants of Citrullus colocynthis on MS medium supplemented with BAP (0.5 mg/l) and NAA (0.5 mg/l). Highest number of shoots (23.0 ± 0.567) was obtained on MS medium containing BAP (0.5 mg/l) and NAA (0.5 mg/l). The regenerated shoots were further elongated on same medium. In vitro shoots were excised from shoot clumps and transferred to rooting medium containing IBA (4.0 mg/l) with 0.2% activated charcoal. The rooted plants were hardened in polycups containing sterile soil and vermiculate and finally well established in the field; survival rate was 60%. This is the first report of direct in vitro plantlet regeneration in Citrullus colocynthis from shoot tip explant.
Pages: 53-56  |  1302 Views  15 Downloads
How to cite this article:
Mahesh Chand Meena, Rishi Kesh Meena and Vidya Patni. High frequency plant regeneration from shoot tip explants of Citrullus colocynthis (Linn.) Schrad. –An important medicinal herb. Journal of Pharmacognosy and Phytochemistry. 2014; 2(6): 53-56.
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