Vol. 3, Issue 1 (2014)
Phytochemical screening, HPTLC studies and screening of antioxidant activity of extracts of leaves of Spathodea campanulata.
Author(s): Maushumi Kulkarni, R G Singhal, Kiran Bhise, Rashmi Tambe
Abstract: Objective: Ayurveda is one of the oldest traditional system of medicine practiced widely in India. Toxicity and side effects of allopathic medicines, has led to sudden increase in the number of herbal drug manufactures. The present study aims at phytochemical screening of methanolic and aqueous extracts of leaves of Spathodea campanulata Family Bignoniaceae. The powdered dried leaves were initially subjected to determination of physical constants. They were extracted with water and methanol. Results: Extracts were subjected to various chemical tests and showed presence of steroids, flavonoids, saponins. Further investigation of the extracts by HPTLC is an attempt to deduce the varied composition of methanolic and aqueous extract of Spathodea campanulata. The antioxidant activity was studied by DPPH assay. Both the extracts showed good antioxidant activity. Conclusion: The extracts showed presence of important phytoconstituents as revealed by the chemical tests and HPTLC chromatograms. Further investigations can be carried out to make formulations of these extracts to treat various disease conditions.
HPTLC of methanolic extract of Spathodea campanulata.10 µl injection.
How to cite this article:
Maushumi Kulkarni, R G Singhal, Kiran Bhise, Rashmi Tambe. Phytochemical screening, HPTLC studies and screening of antioxidant activity of extracts of leaves of Spathodea campanulata.. Journal of Pharmacognosy and Phytochemistry. 2014; 3(1): 08-13.