Author(s):

V. Sivakumar, M. S. Dhana Rajan, A. Mohamed Sadiq, M. Jayanthi

Abstract: A protocol was established for rapid clonal propagation medicinal plant, Tinospora cordifolia (TC),through in vitro culture using nodal explants. Best shoot induction was observed on MS medium with 4.36 µM KIN produces 2.32±0.1 cm length with 1.8±0.1 numbers of shoots with 70% response when compared with other Cytokinins BA and 2iP. After a month of shoot induced culture, creates a phenolic exudation problem in shoot formation, media had discoloration and explants were browning and the buds were break so, further analysis protocols were found by treating with different substances from that, silver nitrate (20%) with KIN (4.36 µM) gives 100% response with 3.01±1.0 cm length of shoots with 2.01±0.1 numbers of shoots within 16 days. Nodal explants of TC were cultured on MS medium with various concentrations of BA alone and in combination with NAA or IAA for shoot proliferation. From that, BA (8.82 µM) alone showed better growth response and produced 4.81±0.2 numbers of shoots with an average length of 3.1±0.1 cm after 20 days. Small shootlet were transferred to shoot elongation medium with 8.82 µM of BA alone. An average length of 4.82±0.4 cm with 4.61±0.2 numbers of shoots produced, 76% of response. The elongated shootlets transferred to half strength MS medium and 6.43 µM of IBA with 3% sucrose produce 5.2±0.2 rootlets per plant with average root length of 3.2±0.1 cm after 27 days. Rooted plantlets were transplanted ex vitro. Approximately 80% of plantlets survived.

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