Author(s):

Ladli kumari, Papiya Mitra Mazumder, Manik, UR Lal and R Dubey

Abstract: Objectives: The present study was aimed at identifying, isolation, antioxidant activity and quantification of a marker compound obtained from M. koenigii leaves. Methods: HPTLC fingerprinting and in-vitro antioxidant activity was carried out for four isolated fractions given the trivial name fraction 1 (MF1), fraction 2 (MF2), fraction 3 (MF3) and fraction 4 (MF4) of methanolic leaf extract of M. koenigii (MEMK). From the HPTLC fingerprint the florescent band (under 350 nm) at Rf: 0.93 (mobile phase n-butanol: acetic acid: water (2:2:4, v/v/v) was found in the fractions of MEMK. So, the florescent band (under 350 nm) at Rf: 0.93 was isolated as marker compound Mk-F4 from MEMK. The marker compound Mk-F4 was quantified by using HPTLC technique. The quantification protocol was followed as per the ICH Q2-R1 guidelines. Results: MF4 showed more effective antioxidant activity as compared to other fractions MF1-MF3 w.r.t. the standard substance used being ascorbic acid in all three models vizDPPH radical scavenging, nitric oxide radical scavenging and hydrogen peroxide scavenging assay may be due to the presence of more percentage of Mk-F4 in MF4. The percentage (W/W) yield of Mk-F4 was found to be 90.0, 95.1 and 16.1% in MF3, MF4 and MEMK, respectively. Conclusion: The present study indicated that M. koenigii and its isolated bioactive compounds be a very useful antioxidant and may form the basis of development of various therapeutic agents with better activity.

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