Vol. 3, Issue 5 (2015)
Evaluation of in vitro study of antioxidant and antibacterial activities of methanolic seed extract of Sesamum indicum
Author(s): Darshika Nigam, Chinky Singh and Udita Tiwari
Abstract: Development of antibiotic resistance in diverse bacterial pathogens has adversely affected antibiotics ability to successfully treat patients and therefore researchers shifted their attention towards herbal products. Therefore, the present study is aimed at identification of secondary metabolites and demonstration of antibacterial and antioxidative activities of methanolic seed extract of black sesame or til (S. indicum). The phytochemical analyses of S. indicum showed the presence of glycosides, flavonoids, alkaloids, tannins and phenols, phytosterols, amino acids and proteins. Total phenolic contents and antioxidant activity were measured in the seed extract was found 19.48+0.98 mg gallic acid per gram of sample and 98.55+3.51% inhibition, respectively. Further, antibacterial activity has been checked in terms of zone of inhibition by disc diffusion method against microorganisms like E.coli and S. aureus for different concentrations of methanolic seed extract which were compared with positive control gentamicin. Sesame seeds showed increasing antibacterial property with an increase in the extract concentration. Methanolic seed extract of S. indicum at concentration 500 mg/ml showed a maximum diameter of inhibition zone for S. aureus 13+0.871 mm, while for E. coli maximum zone of inhibition observed was 10.17+0.946 mm.rnThese results suggest that methanolic seed extract of black sesame contained varied types of pharmacologically active compounds with antioxidant and antimicrobial activities.
How to cite this article:
Darshika Nigam, Chinky Singh and Udita Tiwari. Evaluation of in vitro study of antioxidant and antibacterial activities of methanolic seed extract of Sesamum indicum. Journal of Pharmacognosy and Phytochemistry. 2015; 3(5): 88-92.