Author(s):

Priyanka Sarkar, Murshida Begum, Koly Aktar, Fatema-Tuz-Zohora, Abdul Jabbar, Choudhury Mahmood Hasan

Abstract: The study was aimed at to evaluate the efficacy of relatively polar fraction of Couroupita guianensis Aubl. stem bark on free radical scavenging and brine shrimp lethality. The dried stem bark of Couroupita guianensis Aubl. was cold extracted with methanol and the crude extract obtained was subjected to vacuum liquid chromatography from which the portion eluted with 100% methanol and polar solvents was partitioned with petroleum ether (PEF), carbon tetrachloride (CTF), chloroform (CHF) and water (AQF). These were then subjected to investigation for DPPH free radical scavenging activity and cytotoxicity measurement by Brine shrimp lethality bioassay. From the results it was evident that for cytotoxicity measurement, chloroform fraction (CHF) having lethal concentration of 3.0439 µg/ml has significant activity than other fractions on brine shrimp lethality bioassay and the maximum scavenging activity of aqueous fraction (AQF) was highest, 22.401 µg/ml and petroleum ether fraction (PEF) having a value of 30.0367 µg/ml, showed better activity compared to other fractions. From the findings it was revealed that the polar fraction of the plant extract possesses cytotoxic activity and could be good source of cytotoxic compounds and the promising effect on free radical scavenging suggests that it may play an important role as potential natural source against free radical associated disease.

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