Vol. 4, Issue 4 (2015)
Rapid and highly competent shoot regeneration of Pigeon pea (Cajanus cajan) using variable explants by in vitro culture system
Author(s): Ramdas V Raut, Gaurav A Dhande, Jaysing C Rajput, Arun G Ingale
Abstract: Pigeon pea is legume crop play a crucial role as source of dietary protein in diet, growing extensively in the rainfed and dryland spots of India and worldwide. Plant tissue regenerate through in-vitro system attempting organogenesis as well as embryogenesis pathway, which are in support of unfamiliar genes assimilation targeted for development of transgenic plants. Present study was undertaken to investigate the most appropriate explant type in Pigeon pea regeneration by virtue of in vitro culture system. Genotype Durga (NTL-30) was breed and used as principal material for regeneration studies. Explants isolation from in vitro elevated germinating 6-8 days old seedlings were used for embryonic axis and cotyledonary node, whereas isolation of Scutellum (IZE) explants from overnight imbibed seed. Isolated explants were cultured on Murashige and Skoog medium supplemented with zeatine (0.572, 1.35, 1.47, 2.32 µM) and kinetin (0.46, 0.93, 1.39 & 1.86 µM) as plant growth regulator for regeneration. Additionally medium incorporated with 2.94 µM of silver nitrate to accelerate growth. Successive to regeneration, shoots bud originated from embryonic axis, cotyledonary node and Scutellum were rapidly elongated on MS medium included 0.50 µM gibberlic acid and further rooted on M.S. medium cantoning idol- acetic acid 0.57 µM which notice 95% efficient root induction. The methodology reported here is very simple, skilled and reproducible, can be employing diagonally to corresponding genotypes of pigeon pea, thus can be practically useful for the establishment of transgenic plants.