Author(s):

Minky Mukhija, Mahendra Pal Singh, Kanaya Lal Dhar, Ajudhia Nath Kalia

Abstract: Zanthoxylum alatum is used in traditional medicinal systems for a number of diseases like diarrhoea, fever, toothache, inflammation, headache, microbial infections, cancer, etc. The aim of the present study was to evaluate the ethyl acetate extract of Zanthoxylum alatum stem bark for its cytotoxic and antioxidant potential and to isolate the bioactive constituent(s). Cytotoxicity of ethyl acetate extract was studied on different cancerous cell lines such as pancreatic, lung, breast and colon cancer using MTT assay. In vitro antioxidant potential was evaluated using DPPH, Nitric Oxide scavenging assay and ferric reducing power assay. The estimation of total phenolic compounds in the extract was determined by Folin-Ciocalteu’s method. Isolation of compounds from ethyl acetate extract was done on silica gel column. Structure elucidation was done by using various spectrophotometric techniques like UV, IR, NMR and MS spectroscopy. All statistical analysis was conducted using Graph Pad Prism software. Ethyl acetate extract of plant has shown significant cytotoxic potential on lung and pancreatic cancer cell lines and also shown antioxidant potential. Flavonoids; apigenin and kaempferol-7-O-glucoside have been isolated from column and has shown significant cytotoxic potential. Flavonoids isolated from ethyl acetate extract were responsible for cytotoxic activity of the extract. Hence the Zanthoxylum alatum can be further explored for the development of anticancer drug.

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