Author(s):

Ashwathanarayana R, Raja Naika

Abstract: Olea dioica Roxb.(F.: Oleaceae), an important ethno-medicinal tree, grows in open, evergreen to semi-evergreen and moist deciduous forests up to 1200 m and is distributed throughout the Western Ghats, India. The plant parts such as roots and leaves are used in siddha medicine to cure cancer and for treatment of snake bite. For preliminary phytochemical analysis and antimicrobial assay, bark samples were collected, shade-dried for 21 days and subjected for soxhlet extraction. Polarity wise the solvents used to extract the crude compound are, petroleum ether, chloroform and methanol. In the preliminary phytochemical analysis, petroleum ether showed negative results and chloroform crude extract shows positive result for Tannins and flavonoids. The methanolic crude extract showed positive reaction for Saponins, Flavonoids, Steroids, Glycosides, Phenols and Sterols. The bark crude extract of Olea dioica,. was screened against four pathogenic fungal strains and nine pathogenic bacterial strains by zone inhibition test. For fungal pathogens all extract shows nil effect so that the bark crude extract was no effect on tested fungal pathogens. For bacterial strains the crude extract shows dose dependent inhibition. The maximum inhibition zone observed in methanolic crude extract for Escherichia coli, Staphylococcus aureus, Salmonella typhi, Klebsiella pneumonia, but for pathogenic fungal strains it showed nil effect. The petroleum ether and chloroform shows negligible antimicrobial activity against almost all tested microbial strains and it also showed that the methanolic crude extract effective against test bacterial strains but not effective against pathogenic fungal strains.

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