Vol. 4, Issue 4 (2015)
Protective role of Bacopa monniera L. against hepatic oxidative stress in wistar albino rats
Author(s): Dharmendra Singh, PV Arya, Neetu Koolwal, Vikram Singh, Ranjana Saxena, MC Sharma, RS Gupta
Abstract: The protective role of ethanol extract of Bacopa monniera against alcohol plus carbon tetrachloride (CCl4) induced hepatic oxidative stress in male wistar albino rats has been evaluated. Hepatic oxidative stress was significantly (P<0.001) induced by 30% alcohol (3.0 ml/100g b. wt/day, po) and carbon tetrachloride (1:1 groundnut oil, 0.5 ml/kg b. wt, ip, on day 20th only) for 21 days. Simultaneously, the oral administration of Bacopa monniera extract, at the doses of 100 mg and 200 mg/kg b. wt /day) for 21 days, significantly (P<0.001) restored the enzymatic activities of gamma glutamyl transpeptidase (γ-GTP), aspartate and alanine aminotransferases (AST, ALT), alkaline phosphatase (ALP), total bilirubin, total protein and albumin in serum. Further, the levels of cholesterol, triglycerides, lipid peroxidation (LPO), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), cytochrome-P- 450 and ascorbic acid in the liver also returned near to the normal level in alcohol plus carbon tetrachloride–intoxicated rats. These biochemical observations were also supported by histopathological findings. In conclusion, Bacopa monniera showed liver protection in wistar albino rats possibly by its free radical scavenging activity along with reduction of fat metabolism might be due to the presence of triterpenoid saponins, alkaloids, total phenolics and flavonoids in the extract and/or the purified compounds- betulinic acid, wogonin and oroxindin, which were isolated from the ethanol extract of Bacopa monniera.
How to cite this article:
Dharmendra Singh, PV Arya, Neetu Koolwal, Vikram Singh, Ranjana Saxena, MC Sharma, RS Gupta. Protective role of Bacopa monniera L. against hepatic oxidative stress in wistar albino rats. Journal of Pharmacognosy and Phytochemistry. 2015; 4(4): 233-241.