Author(s):

Ritu Jha, Rajinder K Gupta

Abstract: Energy drink was prepared from the combination of Aegle marmelos, Rubia cordifolia, Phyllanthus emblica and Beta vulgaris. The ingredients used in the energy drink have very good nutrition as well as pharmacological use. All assays were carried out in the methanolic extract of the sample. Total phenolic, flavanoids and tannin content of the sample were found as 42.25μg GAE/ mg of sample, 101.33µg CE/ mg of sample, 0.5774μg TAE / mg of the sample. Alkaloid content of the sample was found to be 6.18%. The product has good antioxidant activity. DPPH assay % inhibition was found to be 63.88% & FRAP value was found to be 268.58µg BE /mg of the sample. GC/MS screening of sample revealed presence of hexadecanoic acid and octadecanoic acid. Antibacterial activity was done against six bacterial strains, i.e. Salmonella enterica, Staphylococcus aureus, Staphylococcus epidermis, Bacillus cereus, Bacillus subtilis, Escherichia coli. Nutritional analysis of the sample revealed moisture content 11.33%, dietary fibre 14.57%, protein 4.59%, total reducing sugar 8.77%, fat 0.17%. Carbohydrate content was 69.34g/100g and energy content was 266.46 kcal. Heavy metals and trace metals were detected by ICP-OES. Calcium was 75.31 ppm, phosphorus 81.25 ppm, magnesium 56.25 ppm and iron 7.03 ppm. Copper and chromium were present in trace amounts. Physico-chemical analysis revealed pH of the sample was 4.9, acidity was found to be 0.104% and TSS was found to be 26.2 ̊ Brix. Energy drink prepared were subjected to sensory analysis using 9 point hedonic scale and the results showed that it was acceptable to consumers in terms of colour, flavour, taste, aroma, mouth feel and solubility.

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