Author(s):

T Janaki, BK Nayak, T Ganesan

Abstract: Totally 25 actinomycetes were isolated by dry heat (70 oC) pre-treatment method on Starch casein agar media, from the soil sample that was collected nearer to the root region of the mangrove Avicennia marina from the back water area, Ariyankuppam, Puducherry (UT). All the 25 actinomycetes were subjected for primary screening against the 10 gram negative bacteria, 2 gram positive bacteria by Agar plug method. The total percentage of inhibition by actinomycetes against bacteria in primary screening was noted as E.coli-8%, K. pneumoniae-4%, P.vulgaris-4%, P.aeruginosa-24%, S.typhi-4%, S.flexneri-16%, V.cholera-4%, B.bronchiseptica-52%, P.fluorescens-0%, E.faecalis-8%, B.subtilis-40%, S.aureus-8%. Totally 20 (80%) of actinomycetes showed antibacterial activity towards any one of the tested bacteria, 5 (20%) actinomycetes showed no antagonistic activity. Only 2 actinomycetes were selected from A.marina and that were subjected for secondary screening. Broad spectrum antibacterial activity was confirmed by cross streak method for selected antagonistic actinomycetes.

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