Author(s):

Jinku Bora, Sourav Chakraborty, Charu L Mahanta

Abstract: Microbes are omnipresent in nature and soil is the favourable nourishing flora and fauna for the growth of the microbes. In the present study, soil samples were collected from hospital nearby areas and pathogenic bacteria were isolated and identified. The three isolates were cultured and two of them were identified to be as Micrococcus spp. and other one as Pseudomonas spp. Three Indian medicinal plants: Ocimum sanctum, Azadirachta indica and Glycyrrhiza glabra were selected to examine the antimicrobial activity against the Micrococcus species and Pseudomonas species using Agar well diffusion method. Methanolic extraction of the plant leaves and roots were done and phytochemical screening of these plants was performed for constituents such as flavonoids, saponins, terpenoids, glycosides, tannins, anthraquinones, alkaloids, etc. Among all the plants Azadirachta indiaca showed maximum antimicrobial activity (Minimum Inhibitory Concentration ranged from 0.05-0.1 mg/ml) against the isolated microbes. However, the isolated microbes were susceptible to the methanolic extracts of the above mentioned plants. In future, these plants can be further subjected to isolation of the therapeutic antimicrobials for health purposes and food applications.

Pages: 63-66  |  2870 Views  453 Downloads

Download (347KB)