Pharmacognosy

Journal of Pharmacognosy and Phytochemistry

  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal
Login

NAAS Rating: 5.21

updates
NAAS Rating: 5.21 new

Journal of Pharmacognosy and Phytochemistry

Vol. 5, Issue 3 (2016)

Alpha amylase inhibitory activity and in vitro glucose uptake in psoas muscle and adipose tissue of male wistar rats of leaf methanolic extract of Achyranthes aspera

Author(s): N Srinivasulu, P Mallaiah, G Sudhakar, B Sasi Bhusana Rao and D Sarala Kumari
Abstract: The aim of the study was to investigate the anti diabetic activity by determination of the alpha amylase inhibitory activity and in vitro glucose uptake in isolated psoas muscle and adipose tissue of male wistar rats. The plant used in this study, Achyranthes aspera, is traditionally an important herb that possesses therapeutic properties used as medicine in India to cure many diseases. This plant was used in all the traditional system of medicine, Ayurveda, Unani, and Sidha from the ancient time in India. 180 g male wistar rat was sacrificed for isolation of psoas muscle and adipose tissue. 250 mg of tissue was used for this study. The inhibition caused by 0.5% Mercuric chloride was arbitrarily fixed as 100% inhibition. Achyranthes aspera leaf methanolic extract (AALMEt) (5- 25 mg/mL) was showed 29.75 – 71.97% amylase inhibitory activity. In vitro glucose uptake studies were carried out in both psoas muscle and adipose tissue in different sets i.e tissue alone, with insulin (25 mU/L & 50 mU/L), plant extracts (50, 100 & 150 mg/mL), plant extracts along with insulin. Psoas muscle was showed increased absorbance from 50 to 100 mg/mL and decreased absorbance from 100 to 150 mg/mL of plant extract alone and in the presence of insulin too but in adipose tissue glucose uptake was decreased with increased plant extract concentration in plant extract alone and along with insulin too.
Pages: 176-180  |  1126 Views  45 Downloads
How to cite this article:
N Srinivasulu, P Mallaiah, G Sudhakar, B Sasi Bhusana Rao and D Sarala Kumari. Alpha amylase inhibitory activity and in vitro glucose uptake in psoas muscle and adipose tissue of male wistar rats of leaf methanolic extract of Achyranthes aspera. Journal of Pharmacognosy and Phytochemistry. 2016; 5(3): 176-180.
Call for book chapter
Journals List Click Here Research Journals Research Journals
Journal of Pharmacognosy and Phytochemistry