Vol. 5, Issue 3 (2016)
Identification and analysis of major street fast food borne pathogenic fungi and its sensitivity to nystatin
Author(s): Salauddin Al Azad, Sayeed Shahriyar, HM Iftekhar Alam, Kanak Jyoti Mondal and Md. Abdullah-Al-Mamun
Abstract: Street foods consuming is available in Bangladesh, mainly in tea stalls and among the fast foods bread, pates, salty biscuit, cake, sweet biscuit, chatpati and pickle are popular. As these popular foods are available at street side, these are prone to have microbial attack. This study was conducted to determine the presence of pathogens responsible for food spoilage by inoculating samples into Potato-Dextrose-Agar (PDA) media. Three replications of fungi in every seven days of interval were made using the PDA media. In each replication several types of fungi were obtained. Later on evaluating the intensity of the microbes we undertook pure culture and got Aspergillus flavus, Aspergillus niger and Fusarium spp. generally which are considered as the most frequently growing fungi which were properly characterized. Then anti-fungal sensitivity was tested using an anti-fungal agent Nystat (100,000 units/ml). Aspergillus flavus, Aspergillus niger and Fusarium spp. were collected from pure culture media and it was found that Aspergillus flavus and Fusarium spp. as resistance to the aforementioned anti-fungal agent while A. niger remained sensitive to that agent. Isolation of major typical toxins mainly the aflatoxins and analysis of their molecular modeling using bioinformatic tools might be an epoch making event for us in ensuring food safety for all and new drug designing in future.
How to cite this article:
Salauddin Al Azad, Sayeed Shahriyar, HM Iftekhar Alam, Kanak Jyoti Mondal and Md. Abdullah-Al-Mamun. Identification and analysis of major street fast food borne pathogenic fungi and its sensitivity to nystatin. Journal of Pharmacognosy and Phytochemistry. 2016; 5(3): 187-191.