, belongs to family Cucurbitaceae is a perennial herb and a common vegetable referred to as ‘pointed gourd’. It was reported to have several pharmacological properties, however, critical search of literature reviews did not yield any articles that evaluated the phyto-constituents of the aqueous root extract and evaluated its biological properties, therefore, the present investigation was undertaken.
Qualitative analysis of the aqueous extract revealed presence of flavonoids, alkaloids, reducing sugars. The presence of alkaloids and flavonoids were more when compared to other compounds. The UV-VIS profile of root extract revealed peaks at 520nm, 512nm, 505nm, 496nm, 487nm, 483nm, 444nm, 426nm and 222.5nm with the absorption 0.071, 0.083, 0.077, 0.087, 0.090, 0.161, 0.177, 0.268 and 3.996. Further analysis by GC-MS revealed presence of 11 prevailing compounds, such as eicosane 2-methyl (18.16), octadecane (3.68), methoxy acetic acid (0.71), 2 methylhexacosane (16.18), heptacosane (5.85), octacosane (2.53).Antibacterial activity of root extract (water) against one Gram negative bacteria Proteus mirabilis
and one Gram positive bacteria Bacillus subtilis
showed a clear zone of inhibition and the zone of inhibition of Gram positive bacteria was slightly greater (13.7 mm) than that of the Gram negative bacteria (12.1mm); against streptomycin, a broad spectrum antibiotic as control which was statistically significant.
Trichosanthes dioica may be considered as an important plant with antimicrobial property due to presence of various phyto-constituents. We would encourage others to confirm and refute our findings.