Author(s):

Adesalu TA, Temenu TO and Julius ML

Abstract: In this study, the species of Oedogonium was subjected to two weeks batch culturing using Bold’s Basal Medium (BBM).The DNA of the pure culture obtained was extracted using DNeasy® Plant Mini Kit following the protocols, conserved DNA regions of chloroplast genome corresponding to SSU/18S rDNA and Rbcl were amplified and Polymerase Chain Reaction (PCR) product was sequenced. Based on morphology and DNA barcoding, isolates were identified as Oedogonium tenerum for SSU/18S rDNA and Oedogonium cardiacum Wittrock ex Hirn (Chlorophyta) for Rbcl. Lipids analysis and the active compounds of Oedogonium strain were evaluated using Agilent 7890A Gas Chromatography (GC) system and Agilent 5975C inert Mass Spectrometer Detector. Analysis of the sample lipids showed 12.98% lipid content while GC-MS analysis of hexane extract of Oedogonium strain (KU680468 (SSU/18S rDNA); KU680573 Rbcl) revealed the existence of Eicosane, 1-Nonadecene, Tricosane, Heneicosane and Nona hexacontanoic acid adding up to 70.27%. The minor constituents include Butylated hydroxytoluene, Nonadecane, 1-Docosene adding up to 29.3%. The importance of some of these components in medicine includes anti-viral and anti-oxidant properties. Other importance of these hydrocarbons are found in food preservatives and majorly in the petrochemical industry for the production of candles, biopesticides (insect pheromones), paraffin wax and other by-products of petroleum. The result implied a closely related but unique strain which is probably endemic to West Africa and Nigeria in particular as other strains are from North America.

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