Vol. 6, Issue 2 (2017)
Morphological metabolic and biochemical characterization of bacterial root endophytes associated with brown sarson (Brassica rapa L.)
Author(s): Shahid Ahmad Padder, GH Dar, Zahoor Ahmad Bhat, Kuldeep Verma and Amir Bashir Wani
Abstract: In the present investigation root samples of brown sarson (Brassica rapa L.) were collected from three districts of Kashmir valley viz., Anantnag, Srinagar and Baramulla. The bacterial population per gram of all the root sample was determined and it was observed that one of the site Hugam in the District Anantnag had the highest bacterial population of 4.35 × 105 cfu/g of fresh weight) and site Nowgam in the District Srinagar had the lowest bacterial population of 6.8 × 103 cfu/g of fresh weight). A total of 81 morphologically dissimilar isolates were selected and characterized on Gram’s staining, cell and colony morphology basis and it was observed that Gram negative bacteria formed the dominant group. The colony characterization revealed that circular forms dominated, likewise the colonies with entire margins and convex elevation dominated among all the isolates. The metabolic properties of isolated bacterial root endophytes revealed that all the isolates can metabolize glucose and galactose but some isolates did not metabolize maltose and sucrose respectively. All the isolates were screened for indole acetic acid (IAA) production and it was observed that 44 isolates among all produced IAA with the average production of 8.15 µg/mL.
How to cite this article:
Shahid Ahmad Padder, GH Dar, Zahoor Ahmad Bhat, Kuldeep Verma and Amir Bashir Wani. Morphological metabolic and biochemical characterization of bacterial root endophytes associated with brown sarson (Brassica rapa L.). Journal of Pharmacognosy and Phytochemistry. 2017; 6(2): 226-232.