Vol. 6, Issue 4 (2017)
Preparation of Cu-chitosan nanoparticle and its effect on growth and enzyme activity during seed germination in maize
Author(s): Ram Chandra Choudhary, Arunabh Joshi, Sarita Kumari, Kumaraswamy RV and Vinod Saharan
Abstract: In present investigation, Cu-chitosan nanoparticle was prepared based on ionic gelation method. Prepared NPs were characterized for physicochemical properties using DLS and FTIR. Characterized NPs were evaluated for seedling growth, physiological and biochemical responses during maize seed germination. In Cu-chitosan NPs treatments, significant higher values of shoot length, seedling vigor index, fresh and dry weight were observed at 0.01-0.16% treated seeds. Similarly, at 0.04-0.12% treatment, root length was recorded maximum among all treatments. However, NPs treatment at 0.01-0.016%, per cent germination and root number were not statistically different but higher than control (water), CuSO4 and bulk chitosan treatments. The activity of α-amylase and protease enzymes were recorded maximum at seventh and fifth days of germination and declined in proceeding days in all treatments. Moreover, in fifth days of germination, the increased activity of α-amylase was observed in NPs at 0.01-0.08% (0.90, 0.90 and 0.94 μmol/min/g dw) as compared to other treatments. Protease activity was reported higher in NPs at 0.01-0.04% (3.81 and 3.94 μmol/min/g dw) in fifth days of germination. Therefore, it can suggested that increased activity of α-amylase and protease is due to Cu-chitosan NPs, which could correlated with starch and protein content for higher seedling growth.
How to cite this article:
Ram Chandra Choudhary, Arunabh Joshi, Sarita Kumari, Kumaraswamy RV and Vinod Saharan. Preparation of Cu-chitosan nanoparticle and its effect on growth and enzyme activity during seed germination in maize. Journal of Pharmacognosy and Phytochemistry. 2017; 6(4): 669-673.