Author(s):

Versha Parcha, Neelam Yadav, Amita Sati, Yogita Dobhal and Navneet Sethi

Abstract: In the current study, Rhododendron arborium Sm flowers were explored for its effect on ischemic heart. Total ethanolic and aqueous extract were prepared and subjected to qualitative and phytochemical studies and were screened for cardioprotective potential i.e., infarction size, release of LDH and CK-MB. Increase in infarction size, decrease in release of LDH and CK-MB are parameters for good cardioprotective action. Total ethanolic extract is found to be rich in carbohydrates, glycoside, flavanoids and saponins while total aqueous extract is composed of carbohydrates, glycosides, amino acids, tannins & saponins. Total ethanolic extract significantly attenuated ischemia reperfusion induced increase in myocardial infarct size, decrease in release of LDH and CK-MB coronary effluent as compared to aqueous extract at the dose level of 70mg/mL IP. Standard drug used for the study was Enalapril 2.5mg/mL. Thus, from above results it was observed that total ethanolic extract of flower of Rhododendron arborium Sm has good cardioprotective effect as compared to aqueous extract which is sold in the market as squash. Further ethanolic extract was fractionated with petroleum ether, dichloromethane, n-butanol in search of active principle and screened. For above said parameters, among all the extracts n-butanol showed the maximum cardioprotective potential and can thus result in isolation of active principle from the same.

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