Vol. 6, Issue 5 (2017)
In vitro Screening of Temperature stress tolerance of Rhizobial and Pseudomonas fluorescence isolates
Author(s): K Manasa, R Subhash Reddy and S Triveni
Abstract: A major problem in rainfed agroecosystems is predominance of abiotic stresses like high temperature, salinity, and drought where the survival of bio inoculants is a problematic issue. The variations in results from laboratory to field are more compounded due to various abiotic stresses that prevail under field conditions for a microbial inoculant to establish and to show the desired effect. Such problems can be overcome by screening programme for efficient stress tolerant isolates. This study investigates the impact of temperature on Rhizobial and Pseudomonas flourescence isolates. Fifteen Pseudomonas fluorescence and 15 Rhizobium strains were isolated from rhizospheric soils of student farm and college farm, College of Agriculture, Rajendranagar, PJTSAU, Hyderabad, Telangana state with a view to screen out temperature tolerant isolates. Selected isolates were biochemically characterized also evaluated for their plant growth promoting traits and tested for their tolerance to different temperatures such as high temperature (28 0C, 37 0C and 45 0C), using respective broth under in vitro conditions. After screening for above conditions result showed that two Rhizobial isolates RR-1, GNR-1 and Pseudomonas flourescence isolate i.e GGP-1 isolate tolerant to all conditions of temperature. The data obtained in the present study suggest that RR-1, GNR-1 and GGP-1 would be ideal organisms for further study in pot culture and field experiments to exploit their PGPR potential for a good biofertilizers production.
How to cite this article:
K Manasa, R Subhash Reddy and S Triveni. In vitro Screening of Temperature stress tolerance of Rhizobial and Pseudomonas fluorescence isolates. Journal of Pharmacognosy and Phytochemistry. 2017; 6(5): 764-767.