Author(s):

Jamilah Ali Ahmed Zaed and Poonam Singh

Abstract:
Microbes are diverse and ubiquitous organisms that play a key role as drivers for ecosystem processes and effected on all living including fish, plant, animal and human also, few of the microorganisms present in nature have been cultured and describe. It is generally accepted that less than 1% of bacteria and fungi, present in most homeland have been cultivated for study in pure culture. In the recent years the development of genomics and introduced new techniques and greater possibilities in the study of microbial diversity in the environment, and those techniques metagenome. This research was done on bacterial diversity in water samples of Ganga river in India by using Metagenomics, new technology in the world of genetic engineering field. This study was based on DNA sequences classification or phylogenetically depended on a single gene, 16S RNA (rRNA) gene. The sequence of nucleotides DNA aligned with Bioinformatics tool. It was observed in this study that metagenomics is a good technique for isolation of microbial DNA from water sample by crossing culture process. which show good amount of DNA concentration. That amount of DNA concentration was amplified and sequenced with 16s r RNA. BLAST and phylogenetic tree analysis was done to obtain the relation between types of bacterial sequence analyze in the research. It was observed that The first sequence CG20160320A shows similarity with Bacillus sp.c-2-33,Second sequence CG20160320B shows similarity with Bacillus anthracis strain BHR1P1B3, Third sequence CG20160320C shows similarity with Pseudomonas aeruginosa strain AT cc and Four sequence CG2016032OD shows similarity to Escherichia coli strain ZH193. First type strain is not toxic and second, third and fourth types were toxic especially Bacillus anthracis strain BHR1P1B3.

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