Abstract:
The pathogenicity test of
Xanthomonas axonopodis pv.
punicae was performed with three different methods viz. Pin-prick method, Infiltration method and Spray inoculation method. Data revealed that amongst three different methods, infiltration method was found most suitable for development of symptoms which had taken 7 days for symptoms development. This method was followed by pin prick and spray inoculation method method which had taken 12 and 15.87 days, respectively for initiation of symptoms. Morphological characters of different
Xap isolates were studied on nutrient agar media. Five isolates collected from different locations differed in respect of size of colony, shape of colony and colour of bacterial colony. Isolates
Xap-1 and
Xap-4 developed medium to large bacterial colonies. The isolates
Xap-1 and
Xap-3 developed small to medium sized bacterial colonies, whereas,
Xap-5 produced small bacterial colonies.
Xap-1 and
Xap-3 produced small circular colonies, whereas,
Xap-2 and
Xap-4 produced circular to irregular bacterial colonies and
Xap-5 produced circular bacterial colonies.
Xap-1 and
Xap-4 showed yellowish bacterial colonies, whereas,
Xap-2 and
Xap-5 showed light yellow coloured colonies and
Xap-3 showed yellow coloured bacterial colonies.
Patil AG, Ambadkar CV, Kashid VS and Navgire KD. Standardization of methods for pathogenicity of pomegranate bacterial blight caused by Xanthomonas axonopodis pv. punicae. J Pharmacogn Phytochem 2017;6(5):1763-1765.