Vol. 6, Issue 6 (2017)
Genetic Diversity in Cowpea (Vigna unguiculata (L.) WALP.), Using RAPD Markers
Author(s): Nagalakshmi RM, Kavitha Mary J, Ananda Kumar CR and Usha Kumari R
Abstract: Random amplified polymorphic DNA (RAPD were assayed to determine the genetic diversity of 13 diverse geneotypes of cowpea cultivated in different regions of India. A total of six random primers were used in the study. Amplification of genomic DNA Indian cowpea genotypes with these RAPD primers yielded 39 fragments that could be scored, of which 34 were polymorphic. Number of amplified fragments with random primers ranged from 1 (OPA 06) to 10 (OPA 1). Percentage polymorphism ranged from 71.42% (OPA 12) to a maximum of 100% (OPA 6, OPA 13 and OPA 19), with an average of 88.5%. The Jaccard’s similarity indices based on RAPD profiles were subjected to UPGMA cluster analysis and genotypes grouped in two major groups. five out of 13 genotypes grouped to cluster I and the rest eight to cluster II. The details of diversity analysis of the cowpea genotypes are discussed in the present study.
How to cite this article:
Nagalakshmi RM, Kavitha Mary J, Ananda Kumar CR and Usha Kumari R. Genetic Diversity in Cowpea (Vigna unguiculata (L.) WALP.), Using RAPD Markers. Journal of Pharmacognosy and Phytochemistry. 2017; 6(6): 1632-1635.