Abstract:
Dalbergia sissoo is very important medicinal plant possessing several pharmacologically potent chemicals. It belongs to the family Fabaceae. Woody bark contains various chemicals that make it antihelmintic, antipyretic and analgesic. It is reported to possess various phytochemicals such as norartocarpotin, stigmasterol, neoflavonoids, flavnoids, dalbergichromene cinnamylphenols, 4-phenylchromene, dalbergichromene, 4-phenyl chromene, dalbergichromene, chalcones, isosalipurposide, amino acids, fatty acids, dalbergin and dalbergenone. The biological activity of
D. sissoo is because of compounds such as flavones, isoflavones, quinines and coumarins. Methanolic extract of
D. sissoo stem was mixed with silica gel (60-120 mesh) and subjected to column chromatography to carry out isolation of the compounds from stem of
D. sissoo. Chromatographic separation was carried out over silica gel (60-120 mesh) column and eluted with the solvents of increasing polarity. The column chromatography afforded two compounds i.e. Dalbergin, 2,5-Dihyroxydalbergiquinol.