Author(s):

Sweekruti Barpanda, Sashikala Beura, Sandeep Rout, and Prema Narayan Jagadev

Abstract:
An Investigation has been conducted on in vitro regeneration of Santalum album Linn from Leaf disc explant at the Biotechnology-cum-Commercial Tissue Culture Center, OUAT, Bhubaneswar, during the year 2015-16.The results revealed that leaf discs treated with 0.1% HgCl₂ for 6 minutes was the best treatment for sterilization as it effectively reduced the percentage of fungal contamination (3.33), bacterial contamination (3.33), higher percentage of aseptic culture (93.33) and high survival percentage (86.67). It reduced the days callus initiation (8.33) with good callus spread. On subculture of the calli mass on MS medium fortified with different combinations of BAP (1.5 mg/l, 2.0 mg/l, 2.5 mg/l), NAA (0.5 mg/l), IAA (0.5 mg/l) no further development of has been recorded and the calli mass turned brown and died in the due course of time. Hence, it needs further research for obtaining quality plantlets in Santalum album Linn.

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