Pharmacognosy

Journal of Pharmacognosy and Phytochemistry

  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal
Login

NAAS Rating: 5.21

updates
NAAS Rating: 5.21 new

Journal of Pharmacognosy and Phytochemistry

Vol. 7, Issue 2 (2018)

Bioactive proteins from Plectranthus barbatus and detection by RP-HPLC-PDA

Author(s): Alcides Alves de Freitas, Nerilson Marques Lima, Ildenício Viana Santos, Sandra Bertelli Ribeiro de Castro, Priscila dos Santos Pereira, Lhorrana Lima Santos, Jairo Lisboa Rodrigues, Caio Cesar de Souza Alves and Alessandra de Paula Carli
Abstract: Plectranthus barbatus Andrews (Lamiaceae) is a popular medicinal plant used to treat gastrointestinal and hepatic ailments. In African countries, P. barbatus is widely used as an herbal remedy to manage HIV/AIDS and related conditions. This work describes the extracts phytochemical analysis to extraction of proteins and evaluation of their cytotoxic activity against A549 e RAW264.7 cancer cells, NO production and cell viability from crude extracts and protein fractions as well as detecting the presence of these proteins by RP-HPLC-PDA and spectrophotometry. The presence of proteins was analyzed by electrophoresis in SDS-polyacrylamide gel PAGE12% and detected by HPLC-PDA and spectrophotometry. In the cell viability assay, it can be classified as non-cytotoxic. The aqueous extract from P. barbatus leaves presented cytotoxic action against lung carcinoma tumor cell lines and the aqueous extract did not increase the NO production in relation to the control. These results may contribute to improve the use of this plant by the population in the prevention of diseases, since this species does not present significant toxicity and showed cytotoxicity against lung carcinoma cells.
Pages: 305-309  |  772 Views  32 Downloads
How to cite this article:
Alcides Alves de Freitas, Nerilson Marques Lima, Ildenício Viana Santos, Sandra Bertelli Ribeiro de Castro, Priscila dos Santos Pereira, Lhorrana Lima Santos, Jairo Lisboa Rodrigues, Caio Cesar de Souza Alves and Alessandra de Paula Carli. Bioactive proteins from Plectranthus barbatus and detection by RP-HPLC-PDA. Journal of Pharmacognosy and Phytochemistry. 2018; 7(2): 305-309.
Call for book chapter
Journals List Click Here Research Journals Research Journals
Journal of Pharmacognosy and Phytochemistry