Author(s):

Pooja Barthwal and Deepti prabha

Abstract: The experiments were carried out with an objective of studying the effect of hormonal priming treatments on germination and seedling growth on aged seed of Phaseolus vulgaris variety Pant - Anupama. We collected the seed from Garhwal seed store, Srinagar Garhwal. The laboratory experiments were conducted on blotter at 25⁰C and 30⁰C temperature respectively. We have taken IAA, kinetin and GA₃ with three concentration 5ppm, 10ppm, and 15ppm. The planting value parameters assessed were germination %, speed of germination, germination value, root length, shoot length, seedling length, seedling fresh weight, seedling dry weight, seedling vigor index -1 and seedling vigor index-2. For accelerated ageing seeds were treated with 1% solution of sodium hypo chloride for 5min. After that seeds placed in desiccators for 7 days at 45⁰c for accelerated ageing. Each alternate day we take 20 seed and placed in germinator at 25⁰c. After that seeds were primed with different doses of growth regulators, which were GA₃, IAA and kinetin. Seeds primed with 5 ppm solution of GA₃ showed the highest germination percentage, speed of germination, germination value, root length, shoot length, seedling length and seedling vigor index-1 and vigor index -2. Growth regulators have been used to reduce the affect and to initiate the process of invigoration. This indicates that the lower concentration of growth regulators favors the increased enzymatic activity which leads to the favorable environment for the germination. Marked reduction in germination and other traits might have been due to solute leakage and lipid per oxidation which limit the necessary material for germination and seedling growth. This present study showed that growth regulator in higher concentrations inhibits the seed germination. It can be concluded that significant variation was found in GA₃5ppm.

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