Author(s):

SK Chand Basha, KRS Sambasiva Rao, E Venkatanagaraju and FR Jobi Xavier

Abstract: Mangroves are the versatile and indomitable plant community for a wide range of bioactivities owing to its unique marine - terrestrial extreme interface. The objective of the current study is to evaluate the hypothesis, to what extent the geography (distance from the shore) influences the α-Amylase production from mangrovian actinomycetes. Six different soil samples (A₁, A₂, A₃, A₄, A₅ and A₆) were collected from Coringa mangroves based on shore to distant shore and were subjected to serial dilution and isolation techniques. A total of nine strains were identified based on colony characteristics. The primary screening for α Amylase recognized two strains (ANUKCJV1:132135 – A₅ and ANUKCJV2:130305 – A₂) as the potential candidate strains and were subjected to α-Amylase production, the two strains exhibited 0.491 and 0.303 IU of α-Amylase activity. The two potential strains were identified as Streptomyces pluripotens and Streptomyces chilikensis respectively based on 16S r DNA sequencing. The sequences of the two potential strains were deposited in the NCBI with accession codes; ANUKCJV1 – MH973320 and ANUKCJV2 – MH973321 respectively. Based on the current study it may be concluded that both the extremeness and favourability of the mangrovian environ influences the α-Amylase production.

Pages: 775-783  |  1311 Views  312 Downloads

Download (942KB)