Vol. 9, Issue 3 (2020)
Phytochemicals, total lipids and molecular characterization of West African strain of Oscillatoria sp. (Cyanobacterium) isolated from Ceratophyllum demersum L. (Hornwort)
Author(s): Adesalu TA and Kuti FO
Abstract: Cyanobacteria are potential sources of biologically active compounds with antiviral, antibacterial, antifungal, and anticancer activities. Bioactive compounds were obtained from the batch culture using Wright’s Cryptophyte (WC) medium for Oscillatoria sp. isolated from Ceratophyllum demersum L. The DNA of the pure culture was extracted using DNeasy® Plant Mini Kit following the protocols, conserved DNA regions of chloroplast genome corresponding to SSU/18S rDNA and Rbcl were amplified and Polymerase Chain Reaction (PCR) product was sequenced. Lipids analysis and the active compounds of Oscillatoria strain were evaluated using Agilent 7890A Gas Chromatography (GC) and Agilent 5975C inert Mass Spectrometer detector. GC-MS analysis of hexane extract of Oscillatoria strain showed the presence of bicyclic sesquiterpene (Caryophyllene), α–Caryophyllene (monocyclic sesquiterpene) and terpenoids (Caryophyllene oxide). The result implied a closely related but unique strain which is probably endemic to West Africa and Nigeria as other strains are from other parts of the world, the potentials of this Oscillatoria strain in pharmaceutical industries is also worthy of note.
How to cite this article:
Adesalu TA and Kuti FO. Phytochemicals, total lipids and molecular characterization of West African strain of Oscillatoria sp. (Cyanobacterium) isolated from Ceratophyllum demersum L. (Hornwort). Journal of Pharmacognosy and Phytochemistry. 2020; 9(3): 18-25.