Vol. 6, Special Issue 1 (2017)
Molecular diversity in Indian isolates of Fusarium oxysporum f.sp. ciceri by issr analysis
Author(s): PN Rakhonde, SS Mane, AD Gawande, RM Wadskar, GF Vyavhare and AD Harne
Abstract: Eighteen isolates of Fusarium oxysporum f.sp. ciceri causing wilt of chickpea representing nine states and eight Agro climatic region of India were analysed for their virulence and genetic diversity. All the isolates proved to be pathogenic to susceptible cv. JG-62. From which, three isolates were found highly pathogenic (71-100%), eight were strongly pathogenic (51-70%) and seven moderately pathogenic (21-50%) to cv. JG-62. The isolates of the pathogen showed high variability in causing wilt incidence on set of twelve host differential cultivars of chickpea namely JG-62, BG-212, L-550, JG-74, CPS-1, WR-315, DCP-92-3, KWR-108, JG-12, Annegiri, IPC-2004-52 and K-850. On the basis of host differential reaction, eighteen isolates could be classified into five races. The same set of isolates was used for molecular characterization with inter simple sequence repeats (ISSR) - polymerase chain reaction (PCR). Unweighted paired group method with arithmetic average grouped the isolates into five categories at a genetic similarities ranging from 50 to 94 per cent.
How to cite this article:
PN Rakhonde, SS Mane, AD Gawande, RM Wadskar, GF Vyavhare and AD Harne. Molecular diversity in Indian isolates of Fusarium oxysporum f.sp. ciceri by issr analysis. 2017; 6(6S): 195-201.