Pharmacognosy

Journal of Pharmacognosy and Phytochemistry

  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal
Login

NAAS Rating: 5.21

updates
NAAS Rating: 5.21 new

Journal of Pharmacognosy and Phytochemistry

Vol. 7, Special Issue 1 (2018)

Temporal and spatial dynamics of Tomato leaf curl disease at Chitrakoot region in India

Author(s): Aniruddha Kumar Agnihotri and Sudhakar Prasad Mishra
Abstract: Tomato is the most important vegetable crop next to potato cultivated across the world. A severe leaf curl disease with puckering, mottling, chlorosis and distorted growth was observed in tomato (Solanum lycopersicum) with significantly high disease incidence (15.2 to 35.9% in summer crop and 12.7 to 31.3% in Rabi crop) at Chitrakoot region during survey 2015-16 and samples were collected. Presence of huge population of whitefly indicated the possibility of begomovirus infection. The causal agent was amplified by PCR (~536 bp and ~1.1 kb) from 102 symptomatic leaves and 63 whiteflies DNA using two set of begomovirus specific degenerate primers. Randomly sequenced four amplicon showed highest similarity (96-99%) with Tomato leaf curl New Delhi virus (KF537780: India: Varanasi). In temporal dynamics study, transplanting of 5th November had minimum disease incidence ranged from 3.3 to 12.2 per cent and low whitefly population than other two early planted crops. A positive linear correlation (y = 2.841x R2 = 0.374) was recorded between whitefly and leaf curl incidence. The study covered survey, molecular diagnosis of Tomato leaf curl disease along with temporal dynamics and development of eco-friendly ToLCD management strategy in Chitrakoot area for the first time.
Pages: 531-536  |  577 Views  3 Downloads
How to cite this article:
Aniruddha Kumar Agnihotri and Sudhakar Prasad Mishra. Temporal and spatial dynamics of Tomato leaf curl disease at Chitrakoot region in India. 2018; 7(1S): 531-536.
Call for book chapter
Journals List Click Here Research Journals Research Journals
Journal of Pharmacognosy and Phytochemistry