Vol. 7, Special Issue 1 (2018)
Quantative analysis of Bt protein on different events of IR-64 plants and insects sample by ELISA
Author(s): Gajendra Kumar, Shanjay Sharma, Girish Chandel and Randeep Kr Kushwaha
Abstract: The experiment was undertaken at the transgenic containment facility, Department of Plant molecular biology and Biotechnology, College of Agriculture, Raipur during 2014 and 2015. The primary exposure data indicated that amount of Bt protein contained transgenic lines fed by BPH ranged from 0.001 to 0.109μg/g and secondary exposure data indicated that the amount of Bt protein contained BPH on transgenic lines fed by Coccinella beetle ranged from 0.005 to 0.044μg/g. On the basis of two years mean, in primary exposure, the maximum (0.106 μg/g) amount of Bt protein was expressed in IR64-4 followed by IR64-2 (0.086 μg/g) and minimum in IR64-C (0.001 μg/g) Whereas, in secondary exposure, the maximum (0.044 μg/g) amount of Bt protein was expressed in IR64-3 followed by IR64-2 (0.029 μg/g while protein was not in detectable level in IR64-C.The transgenic plants expressing insecticidal properties are becoming environmentally safe alternatives to chemical pesticides.
How to cite this article:
Gajendra Kumar, Shanjay Sharma, Girish Chandel and Randeep Kr Kushwaha. Quantative analysis of Bt protein on different events of IR-64 plants and insects sample by ELISA. 2018; 7(1S): 1397-1400.