Volume 3, Issue 5

 

 

Author(s): Asha Bisht, NV Satheesh Madhav, Kumud Upadhyaya

Abstract: Polyherbal formulation was prepared based on the ideal tree concept. Phytochemical screening was performed according to the standard methods. Acute toxicity was performed according to OECD guidelines, using 2000 mg/ kg BW as a limit dose. Anti-hyperlipidemic activity of the aqueous extract of polyherbal formulation (50 mg/kg, p.o.) was determined in Triton X-100 induced (100 mg/kg) hyperlipidemic albino wistar rats and compared against simvastatin (10 mg/kg, p.o,)  as a standard .On 21st day serum lipid levels were estimated by using respective diagnostic kits. Results are expressed as mean ± SD and subjected to One Way Analysis of Variance (ANOVA) followed by Dunnett’s test and values P<0.01 were considered to be significant. Antioxidant activity was determined by using hydrogen peroxide scavenging activity method. HPTLC analysis of the polyherbal formulation was performed using quercetin as a marker compound. Cell viability studies were performed to know the cell viability and for the estimation of IC50 values, by MTT assay on Vero monkey normal kidney epithelial cell lines.
Polyherbal formulation at the dose of 50 mg/kg, lowered TC, TG, VLDL-C, LDL-C levels with simultaneous increase in high density lipoproteins cholesterol (HDL-C) levels (P<0.01). HPTLC analysis showed the presence of quercetin, which was also confirmed by showing significant antioxidant activity as compared to ascorbic acid. Phytochemical analysis also revealed the presence of flavanoids. Cell viability studies showed the IC50 value of >200, indicating the safety profile of the polyherbal formulation. This is the first study which investigates the anti-hyperlipidemic effect at a low dose level of 50 mg/kg BW.

 

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