Author(s):

N Anitha Chandran and PR Unnikrishna Pillai

Abstract: Effects of 0.01% SA (Sodium azide) for 3 hours on secondary metabolites production in Andrographis paniculata was carried out in this study. Calli produced from leaf explants inoculated on Murashige and Skoog’s (MS) medium supplemented with 2 mg/l NAA, 1 mg/l Kinetin and 50 mg/l Phenylalanine. Treatment of 0.01% SA for 3 hours positively influence in secondary metabolites production when compared to the control. The study also showed that 0.01% SA for 3 hours treated samples showed enhanced production in the fresh weight, dry weight of calli and callus induction frequency compared to the control.

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