Author(s):

Ivan Georgiev Ivanov

Abstract: Fragaria vesca L. (wild strawberry) belongs to the Rosaceae family. The aerial part from wild strawberry was applied in traditional medicine. Fragaria vesca are a rich source of biologically active substances like tannins, proanthocyanidins, flavonoids and phenolic acids. The aim of this study was to compare the value of phytochemical compounds and antioxidant activities in infusion, decoction glycerine extracts and tinctures obtained from the F. vesca leaves and flowers. The extracts were analyzed regarding their secondary metabolites content (total polyphenols, total tannins, and total proanthocyanidins) and antioxidant activities (DPPH and FRAP methods). The analysis of 50% glycerin extracts under ultrasound irradiation shown the highest level of total polyphenols (150.2 mg GAE/g dw), total tannins (65.8 mg TAE/g dw) total proanthocyanidins (193.3 mg LE/ 100g dw) and antioxidant activities – radical scavenging activity (DPPH – 1255.2 m mol TE/ g dw) and metal reducing ability (FRAP – 1129.2 mmol TE/ g dw). The results showed that the 50% glycerin extracts from leaves and flowers of Fragaria vesca are appropriate additives for the preparation of emulsion for natural cosmetic products with improved biological activity. The most suitable solvent for tincture preparation was 50% ethanol. With this solvent, the biologically active substances were best extracted (total polyphenols – 10847.3 mg GAE/ L). The influence of the storage period at room temperature on the amount of biologically active surfactants in the obtained tinctures was also monitored. The optimal shelf life for tinctures has been determinate at 12 months.

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