Author(s):

Pushpendra Sharma and Mamta Baunthiyal

Abstract: Pinus roxburghii commonly used as timber plant along with medicinal properties is used in present study as a sustainable approach for utilizing bio-resources. Plant-associated endophytic Actinobacteria were objected and explored for their therapeutic potential properties. The results indicated that a total of two hundred eighty-three endophytes were isolated from different tissues and belonged to predominating genus Streptomyces followed by Kitastaosporia, Actinomadura, Micromonospora, Streptosporangium, Microbiospora and Nocardia. During preliminary screening, 47.7% and 14.8% were active against bacterial and fungal pathogens respectively. Isolate GBTPR-137 having maximum zone of inhibition against Micrococcus luteus (27.3±0.5 mm) followed by Pseudomonas aeruginosa (18.3±0.5 mm), Neisseria cinerea (18.7±0.5 mm) and Klebsiella pneumonia (18.3±0.5 mm) While GBTPR-281 was having antibacterial as well as antifungal activity against multicellular fungi Aspergillus fumigates (16.7±0.5 mm), Microsporium gypseum (12.3±0.5 mm) and A. flavus (14.7±0.9 mm). The results depicted that these plants were a worthy niche for Actinobacteria producing important bioactive secondary metabolites.

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