Author(s):

Patel Kunjankumar F, Dr. YM Shukla, Poonam D Kanani and Pitambara

Abstract: The yield of cumin (Cuminum cyminum L.) is affected by lack of superior varieties, scientific crop production technology and vulnerability to various biotic and abiotic stresses during its life cycle. Defence mechanism allows plant to tackle various stresses. Transcription factors (TFs) are at the center of the complex system controlling cellular growth, differentiation, genetic response to the environment, organism development, evolution and response to various biotic and abiotic stresses. Out of total six primers, four primers namely EF1-α, UBQ_10, ACT-11 and Tip41 showed amplification of the endogenous genes in GC-4 and GC-2 varieties c-DNA template were selected and utilized for validation of endogenous genes through qRT-PCR. Total 33 primer pairs of WRKY transcription factors related genes were screened with DNA of both varieties and 10 primer pairs of WRKY transcription factors related genes were selected and screened with c-DNA sample. After WRKY transcription factors related primers screening with 24 hours shoot tissue c-DNA sample of GC-4 variety, WRKY14_2, WRKY25_1, WRKY27_2 and WRKY58_2 primers were selected for gene expression study through real time PCR.

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