Author(s):

Sreya Dutta, Kalyan Hazra, Deboleena Paria, Shreya Ghosal, Amit Kumar Dixit and Mruthyumjaya Meda Rao

Abstract: The present study aimed to generate the qualitative, quantitative parameters for seeds of Argemone mexicana L. (Family: Papaveraceae) through phyto-pharmacognostical, physicochemical, phytochemical studies. Qualitative chromatographic profiling has been generated. Spectral pattern of the extract by infra-red spectroscopy has also been studied. The pharmacognostical evaluation showed useful characters for the standardization of Argemone mexicana seed. Transverse section showed presence of lignified, non-lignified fibers, scalariform vessels and oil globules in mesocarp region. Lignified stone cells, endosperm and endocarp are observed in powder microscopy. Physicochemical studies of fruit powder shows, total ash (8.79%), and acid insoluble ash (3.16%). Highest extractive value (36.34%) obtained from acetone. Phytochemical analysis revealed the presence several secondary metabolites. Chromatographic profiling done through planar and column and spectral pattern obtained through infra-red. The generated data set by botanical and chemical screening in combination may be considered as a standard for authentication and identification and may be referred in standardisation and monograph preparation.

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