Fingerprinting analysis of the phytosterols from Ailanthus excelsa (Roxb.) leaves using high-performance thin layer chromatography analysis

Sudhir Singh Gangwar; Amita Tilak; Ranjana Sharma; Ravindra C Sutar

Fingerprinting analysis of the phytosterols from Ailanthus excelsa (Roxb.) leaves using high-performance thin layer chromatography analysis

Author(s):

Sudhir Singh Gangwar, Amita Tilak, Ranjana Sharma and Ravindra C Sutar

Abstract:

Objective: The present study was conducted to identify the phytosterols from petroleum ether (PEAE) extract of medicinally and economically useful leaves of Ailanthus excelsa (Roxb.) using High performance Thin Layer Chromatography (HPTLC) technique.

Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp.

Results: HPTLC finger printing of phytosterols of petroleum ether extract of leaves revealedtwelve polyvalent phytoconstituents (12 peaks) and corresponding ascending order of Rf values in the range of 0.019 to 0.928.

Conclusions: With the results of HPTLC analysis and above R_f values we have concluded the presence of phytosterols in the extract.

Pages: 1461-1464 | 948 Views 396 Downloads

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Journal of Pharmacognosy and Phytochemistry

How to cite this article:

Sudhir Singh Gangwar, Amita Tilak, Ranjana Sharma and Ravindra C Sutar. Fingerprinting analysis of the phytosterols from Ailanthus excelsa (Roxb.) leaves using high-performance thin layer chromatography analysis. J Pharmacogn Phytochem 2019;8(5):1461-1464.

Pharmacognosy

Journal of Pharmacognosy and Phytochemistry

Vol. 8, Issue 5 (2019)

Fingerprinting analysis of the phytosterols from Ailanthus excelsa (Roxb.) leaves using high-performance thin layer chromatography analysis

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