Expression, puriï¬cation and bioassay of Cry55Aa protein against tomato root knot nematode, Meloidogyne incognita

A Manivannan; KK Kumar; S Varanavasiappan; S Manimegalai; K Poornima; C Devrajan; D Sudhakar; V Balasubramani

Expression, puriï¬cation and bioassay of Cry55Aa protein against tomato root knot nematode, Meloidogyne incognita

Author(s):

A Manivannan, KK Kumar, S Varanavasiappan, S Manimegalai, K Poornima, C Devrajan, D Sudhakar and V Balasubramani

Abstract:

A nematicidal Cry55Aa protein was expressed and purified from E. coli cells. Higher level of expression was observed in culture induced with 0.1mM of IPTG after four hours of induction. The Cry55Aa protein was purified by resuspending the crude protein in solubilizing buffer at pH 12 for 120 min. When Meloidogyne incognita was tested against different concentration of purified protein, a clear dose response lethality was observed with an LC₅₀ value of 31.4 µg/ml.

Pages: 570-573 | 908 Views 324 Downloads

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Journal of Pharmacognosy and Phytochemistry

How to cite this article:

A Manivannan, KK Kumar, S Varanavasiappan, S Manimegalai, K Poornima, C Devrajan, D Sudhakar and V Balasubramani. Expression, puriï¬cation and bioassay of Cry55Aa protein against tomato root knot nematode, Meloidogyne incognita. J Pharmacogn Phytochem 2019;8(6):570-573.