Manoj Kumar, YP Rajbhar, Vimal Kumar, Lalit Yadav, Jitendra Kumar, Budhesh Pratap Singh and Ajay Yadav

Present investigation Studies on the effect of different surface sterilization agents under in-vitro culture of Strawberry (Fragaria × ananassa Duch.) variety “Chandler” was carried out at the Tissue Culture Laboratory Department of Horticulture, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut during the year 2018-2019. Effect of two different surface sterilization agents’ i.e., mercuric chloride and ethanol were tested on the contamination-free establishment of Strawberry cv. Chandler under in vitro conditions. All the sterilization agents performed better results when used individually for different time intervals. Minimum contamination percentage of explants after 10 days (32.72%) was noted under HgCl₂ 0.1 per cent treating for a period of 2 min. A critical observation was recorded as the maximum survival percentage of explants after 25 days (68.71%) was noted under the treatment of HgCl₂ 0.1 per cent for a period of 2 min. Minimum contamination percentage of explants after 10 days (35.41%) was noted under ethanol 70 per cent treating for a period of 2 min. A critical observation was recorded as the maximum survival percentage of explants after 25 days (70.78%) was noted under the treatment of ethanol 70 per cent for a period of 2 min. The combination of 0.1 per cent Hgcl₂ with 70 per cent ethanol was also found effective for sterilization of Strawberry explants. Best results with lower contamination 0.1 per cent with HgCl₂ for a period of 1 min. (21.76%). Whereas higher explant survival per cent (79.88%) was observed HgCl₂ 0.1 per cent with ethanol 70 per cent for a period of 1 min. The present study concludes that combination use of 0.1 per cent mercuric chloride + 70 percent ethanol for different time intervals was found to be best to generate contamination-free plants in Strawberry cv. Chandler.

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