• Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal
Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 13, Issue 4 (2024)

Anti-inflammatory and antioxidant activities and the thin layer chromatographic fingerprinting of the fruit pulp and epicarp extracts of D. microcarpum

Author(s):

Lateef A Oseni and Boniface Azumah

Abstract:

Extracts from Detarium microcarpum have been reportedly used to manage inflammatory and oxidative stress-related disorders such as rheumatism, kidney pains and skin infections in folkloric medicine. This study focused on the in vitro antioxidant and anti-inflammatory properties of 70% ethanol extracts of the fruit pulp (FPE) and epicarp (FEE) of D. microcarpum. The in vitro antioxidant activity of the extracts was assessed using a DPPH radical scavenging assay, while the anti-inflammatory activity of the extracts was assessed by a protein denaturation inhibition assay. The thin layer chromatography (TLC) mobility profiles of the chloroform fractions of each extract were assessed for fingerprinting. The antioxidant activity of FPE and FEE was concentration dependent, with IC50 values of 252.40 ± 52.50 µg/ml and 115.80 ± 15.70 µg/ml, respectively. Ascorbic acid used as a control in the antioxidant assay exhibited antioxidant activity with an IC50 value of 2.04 ± 0.13 µg/ml. FEE, FPE and Na-Diclofenac also presented significant anti-inflammatory activity, with IC50 values of 1.48 ± 0.45 mg/ml, 1.16 ± 0.22 mg/ml and 0.47 ± 0.15 mg/ml, respectively. Qualitative phytochemical screening revealed the presence of saponins, reducing sugars, phenolic compounds, tannins, flavonoids, and phytosterols in both plant extracts. The FFPE and FEE specimens were partitioned into chloroform. The chloroform fraction of each extract displayed seven (7) bands on the TLC plate with Rf values ranging from 0.77 to 0.051 (for FEE) and 0.92 to 0.14 (for FPE). These findings suggest that the fruit pulp and epicarp of D. microcarpum hold promise as potential sources for the development of novel antioxidant and anti-inflammatory drugs.

Pages: 130-135  |  250 Views  87 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Lateef A Oseni and Boniface Azumah. Anti-inflammatory and antioxidant activities and the thin layer chromatographic fingerprinting of the fruit pulp and epicarp extracts of D. microcarpum. J Pharmacogn Phytochem 2024;13(4):130-135. DOI: 10.22271/phyto.2024.v13.i4b.15002

Call for book chapter