Alang Michael Bong, Beka Robert Germain and Bibila Marylen Mbongeh

From antiquity, nature has been a vital source of medicinal agents. This explains why a large number of natural products is still being used in medical practice. Plants preparations are habitually used in man's and animals' typical diets due to their chemical composition, and medical usefulness. In this study, the phytochemical analysis and antimicrobial evaluations of Tapinanthus bangwensis were carried out on the plant’s leaf extract. The leaf extracts were obtained through maceration and solvent extraction using distilled water and 95% ethanol. The extracts were analyzed using qualitative methods for the detection of secondary metabolites and some quantitative analyses were carried out to estimate quantitative data of phenolic compounds, polyphenols and flavonoids contents of the plant extracts which is an indication of the bioactive potency of the extracts. Antibacterial susceptibility tests were done and Minimum inhibitory concentrations (MIC) and Minimum bactericidal concentrations (MBC) were determined using the agar disk diffusion method and micro-dilution methods respectively. The qualitative results showed the presence of most phytochemicals such as phenols, alkaloids, tannins, flavonoids, saponins, quinones, carbohydrates, and glycosides in Tapinanthus bangwensis leaves while quantitative results showed a significant high density of bioactive molecules in the plant extract. Furthermore, the plant extract could inhibit the proliferation of test organisms. The MIC of T. bangwensis extract against Escherichia coli was 4mg/mL, the MBC was found to be 6 mg/mL and the MBC/MIC ratio is 1.5. This ratio provides valuable information about the bactericidal activity of the extract. This study therefore reveals the presence of vital bioactive molecules the plant T. bangwensis with laudable pharmaceutical, cosmetic and therapeutic applications. Considering the demonstrated antimicrobial potency of the plant extract against E. coli, the plant is therefore a potential and reliable source of natural antibiotics for industrial applications.

DOI: 10.22271/phyto.2024.v13.i4c.15015

Pages: 240-246  |  144 Views  50 Downloads