Sanghadeep Siddharth Ukey and Dayanand Pandurang Gogle

Piper nigrum L. is most extensively used spice all over the world. It consists of major bioactive compound piperine which is an alkaloid. It is widely used in various herbal cough syrups and it is also used in anti-inflammatory, anti-malarial, anti-leukemia treatment. Extraction and isolation of bioactive compound is the new challenge in the field of phytochemistry. The aim of the present study was to evaluate the extractive value of P. nigrum L. in different solvent fractions. Phytochemical analysis of fractions was conducted to detect the presence of various phytochemical constituents. The objective of the study was investigated by studying their total phenol and flavonoid content (TPC and TFC), In vitro antioxidant parameters (DPPH, FRAP, ABTS, DMPD, and TAA). The plant part used for the medicinal purposes have been investigated and compared with synthetic antioxidants, BHA, BHT and ascorbic acid. The qualitative analysis of crude extract and solvent fractions revealed the presence of various phytochemicals of pharmacological significance. The total phenols and total flavonoids in Piper were found to be 178.42±3.29mg/g of GAE and 157.22±5.24mg/g of QE respectively. The highest DPPH scavenging assay showed IC₅₀ value of 21.21±0.297µg. There has been a direct relation between Phenol, flavonoid and DPPH scavenging assay. ABTS assay showed IC₅₀ value as 8.32±0.164µg and DMPD assay showed IC₅₀ value as 59.47±0.470µg. 

DOI: 10.22271/phyto.2024.v13.i4e.15029

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