Emeka S Asiwe, Uchechi E Enenebeaku, Precious N Alisi, Obioma J Eboh and Peter O Opara

The present study investigated the protective effect of binary mixtures of ethyl acetate fractions of Persea americana seed (PAEA) and Bryophyllum pinnatum leaf (BPEA) against non-steroidal anti-inflammatory drug (NSAID) induced oxidative stress in rats. The study comprised of 10 experimental units of four (4) rats each. The control groups were normal control group (NC), untreated control (UC), standard (0.02g kg^-1 body weight Omeprazole (OMEP). Treatment groups included single and binary combinations of PAEA and BPEA, PAEA+ BPEA (1:1), PAEA+ BPEA (1:2), PAEA+ BPEA (1:3), PAEA+ BPEA (2:1), PAEA+ BPEA (3:1). The rats were initially administered 0.4gKg^-1 b.wt. of respective mixtures orally for 3 weeks. Oxidative stress was induced by administration of 0.03 g/kg body weight indomethacin on the 22nd day after overnight fasting. Oxidative markers were determined using reference methods. The results revealed that indomethacin administration resulted in increased malondialdehyde level and significant reduction in stomach tissues GSH concentration when matched with normal control rats. Gastric tissue SOD, Catalase, GST, and GPx activity were significantly (p<0.05) reduced indicating a compromise in antioxidant protection. Administration of PAEA + BPEA (2:1) and PAEA + BPEA (3:1) resulted in significant protection against oxidative changes and restoration of GSH concentration similar to normal control. Binary combinations of the fraction had significantly (p<0.05) higher antioxidant effect than individual plant fractions. The fractions antioxidant protection was in the order PAEA + BPEA (3:1) > PAEA + BPEA (2:1) > PAEA + BPEA (1:1), > PAEA + BPEA (1:2) > PAEA + BPEA (1:3) > PAEA > BPEA.

DOI: 10.22271/phyto.2024.v13.i4e.15033

Pages: 413-419  |  102 Views  40 Downloads