Author(s):

Poulami Gupta and Bratati De

Abstract: Plants have both induced and constitutive mechanisms to resist environmental stress. Different mechanisms of induced responses to tolerate salinity in rice, a staple food for more than half of the world’s population, are in report. The objective of the study was to search for constitutive metabolite(s) for differentiate the grains of salinity tolerant and sensitive rice varieties, on the basis of metabolic profile. Rice grains of total 13 varieties, (8 tolerant and 5 sensitive) were analyzed by gas chromatography coupled with mass spectrometry for identification of aqueous methanol soluble metabolites and cell wall bound phenols. Chemometric study (Orthogonal Partial Least Squares Discriminant Analysis and Partial Least Squares Discriminant Analysis) separated the sensitive and tolerant varieties based on 86 metabolites including 8 wall bound phenols. Four wall bound phenols were identified from the loadings to contribute most for the separation of the two groups, tolerant and sensitive. One cell wall bound phenol 4-hydroxy-3-methoxybenzoic acid (vanillic acid) was detected only in the sensitive varieties but not in the tolerant varieties. This finding suggests that cell wall bound phenol 4-hydroxy-3-methoxybenzoic acid is a constitutive biomarker metabolite to distinguish tolerant and sensitive varieties rapidly.

DOI: 10.22271/phyto.2024.v13.i4f.15044

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